5H38
Structural analysis of KSHV thymidylate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-31 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.518, 80.545, 65.602 |
| Unit cell angles | 90.00, 110.69, 90.00 |
Refinement procedure
| Resolution | 19.827 - 1.700 |
| R-factor | 0.1682 |
| Rwork | 0.167 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hvy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.117 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.695 | 1.700 |
| Number of reflections | 65947 | |
| <I/σ(I)> | 36.5 | 4.8 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | potassium phosphate (monobasic), PEG 8K, glycerol |
