5H1Y
Crystal structure of the DDX41 DEAD domain in complex with SO42- and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.881, 62.746, 92.881 |
| Unit cell angles | 90.00, 94.32, 90.00 |
Refinement procedure
| Resolution | 35.891 - 2.260 |
| R-factor | 0.1883 |
| Rwork | 0.186 |
| R-free | 0.23280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fe2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.234 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Number of reflections | 23367 | |
| <I/σ(I)> | 21.4 | 3.34 |
| Completeness [%] | 98.6 | 97 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2M ammonium sulfate, 25% PEG 3,350, 0.1M Bis-Tris, pH 6.5 |
