5H1L

Crystal structure of WD40 repeat domains of Gemin5 in complex with 7-nt U4 snRNA fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 1 21 1
Unit cell lengths	59.535, 124.790, 61.016
Unit cell angles	90.00, 117.97, 90.00

Refinement procedure

Resolution	40.784 - 2.100
R-factor	0.1971
Rwork	0.195
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h1j
RMSD bond length	0.005
RMSD bond angle	0.936
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.090	0.063	0.533
Rmeas	0.108
Rpim	0.059
Total number of observations	153633
Number of reflections	45763
<I/σ(I)>	7.2
Completeness [%]	99.7	99.1	99.7
Redundancy	3.4	3.3	3.4
CC(1/2)		0.990	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	0.1M Sodium malonate (pH 7.0), 12% PEG 3350