5H1K
Crystal structure of WD40 repeat domains of Gemin5 in complex with 13-nt U4 snRNA fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.654, 107.191, 151.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.17216 |
Rwork | 0.170 |
R-free | 0.20626 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h1j |
RMSD bond length | 0.009 |
RMSD bond angle | 1.249 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.116 | 0.683 |
Number of reflections | 126392 | |
<I/σ(I)> | 17.4 | 2.8 |
Completeness [%] | 99.7 | 99 |
Redundancy | 7.9 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 4% Tacsimate (pH 8.0), 16% PEG 3350 |