5H0Y

Crystal structure of H88Y mutated human transthyretin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	41.349, 62.805, 85.244
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.403 - 1.800
R-factor	0.2055
Rwork	0.203
R-free	0.24330
RMSD bond length	0.005
RMSD bond angle	0.964
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.200
High resolution limit [Å]	1.800
Number of reflections	10536
<I/σ(I)>	22.9
Completeness [%]	99.0
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	2.0 M ammonium sulfate, 25% glycerol, 0.1 M MES