5H0X
Crystal structure of H88S mutated human transthyretin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 42.330, 85.480, 64.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.934 - 1.573 |
R-factor | 0.2403 |
Rwork | 0.240 |
R-free | 0.25310 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.851 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1-2155_1069: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.900 |
High resolution limit [Å] | 1.570 |
Number of reflections | 32905 |
<I/σ(I)> | 20.6 |
Completeness [%] | 99.7 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 1.8 M ammonium sulfate, 25% glycerol, 0.1 M sodium acetate |