5GZK
Endo-beta-1,2-glucanase from Chitinophaga pinensis - sophorotriose and glucose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.106, 117.401, 75.554 |
| Unit cell angles | 90.00, 92.56, 90.00 |
Refinement procedure
| Resolution | 49.460 - 1.700 |
| R-factor | 0.16761 |
| Rwork | 0.166 |
| R-free | 0.19875 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gzh |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.158 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.110 | 0.680 |
| Number of reflections | 120399 | |
| <I/σ(I)> | 13.1 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.696 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | 0.2 M sodium chloride, 0.1 M sodium/potassium phosphate (pH 6.2), 10% PEG 8000 |
