5GZ3

Structure of D-amino acid dehydrogenase in complex with NADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-04
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	63.585, 78.232, 68.969
Unit cell angles	90.00, 107.25, 90.00

Refinement procedure

Resolution	50.000 - 1.590
R-factor	0.1933
Rwork	0.191
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gz1
RMSD bond length	0.030
RMSD bond angle	2.621
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.620
High resolution limit [Å]	1.590	4.320	1.590
Rmerge	0.084	0.046	0.458
Number of reflections	81038
<I/σ(I)>	16
Completeness [%]	93.7	78.5	83.1
Redundancy	6.3	6.1	5.6
CC(1/2)		0.996	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	PEG 8000