5GWB

Hen Egg White Lysozyme native crystals soaked for 2 hours in precipitant solution containing 1 M guanidine hydrochloride and 25% glycerol, before data collection

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-20
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54187
Spacegroup name	P 43 21 2
Unit cell lengths	77.360, 77.360, 36.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.151 - 2.001
R-factor	0.1841
Rwork	0.182
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.008
RMSD bond angle	1.075
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.200	2.290
High resolution limit [Å]	2.000	2.000
Number of reflections	7861
<I/σ(I)>	0.99
Completeness [%]	99.1
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	300	Sodium Chloride