5GVR
Crystal structure of the DDX41 DEAD domain in an apo closed form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.830, 81.830, 69.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 1.500 |
| R-factor | 0.18205 |
| Rwork | 0.181 |
| R-free | 0.20806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fe2 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.182 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 1.510 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.065 | |
| Number of reflections | 41380 | |
| <I/σ(I)> | 18.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | DL-malic acid |
