5GTP
The agonist-free structure of human PPARgamma ligand binding domain in the presence of the SRC-1 coactivator peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-21 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 131.310, 53.062, 53.552 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.21292 |
| Rwork | 0.211 |
| R-free | 0.25809 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vn2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.496 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.603 | |
| Number of reflections | 16193 | |
| <I/σ(I)> | 36.2 | 4 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 5.8 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 296 | 2.2 M sodium malonate (pH 7.0) |
