5GTJ

CRYSTAL STRUCTURE OF CATALYTICALLY ACTIVE FORM OF HUMAN DUSP26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2013-01-24
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97935
Spacegroup name	C 1 2 1
Unit cell lengths	122.617, 100.803, 70.870
Unit cell angles	90.00, 114.67, 90.00

Refinement procedure

Resolution	39.691 - 2.000
R-factor	0.1987
Rwork	0.196
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.910
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.691
High resolution limit [Å]	2.000
Number of reflections	53434
<I/σ(I)>	17.7
Completeness [%]	97.2
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	0.1M Tris-HCl pH 8.5, 0.9M potassium thiocyanate