5GTC

Crystal structure of complex between DMAP-SH conjugated with a Kaposi's sarcoma herpesvirus LANA peptide (5-15) and nucleosome core particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98000
Spacegroup name	P 21 21 21
Unit cell lengths	107.225, 109.017, 176.194
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.590 - 2.700
R-factor	0.214
Rwork	0.211
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3afa
RMSD bond length	0.011
RMSD bond angle	1.306
Data reduction software	Aimless (0.5.17)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.590	48.590	2.760
High resolution limit [Å]	2.690	11.710	2.690
Total number of observations	57172	796	3923
Number of reflections	57172
<I/σ(I)>	12.3	35.7	2.2
Completeness [%]	98.3	98.6	88.4
Redundancy	1	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	potassium cacodylate, potassium chloride, manganese chloride