5GSW
Crystal structure of EV71 3C in complex with N69S 1.8k
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-08 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.501, 70.535, 94.923 |
| Unit cell angles | 90.00, 118.23, 90.00 |
Refinement procedure
| Resolution | 48.620 - 3.190 |
| R-factor | 0.197 |
| Rwork | 0.189 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3osy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.622 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 8.670 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.157 | 0.581 |
| Number of reflections | 17983 | |
| <I/σ(I)> | 7.36 | |
| Completeness [%] | 96.8 | |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 289 | 0.1 M Tris-HCl, pH 7.7, 200 mM Sodium Citrate, 16% PEG 3350. |
