5GQ9
Crystal structure of Thermus thermophilus Argonaute in complex with g1C siDNA and DNA target
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97848 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.831, 116.503, 161.024 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.130 - 2.700 |
R-factor | 0.1919 |
Rwork | 0.189 |
R-free | 0.23660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ncb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.310 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.542 | |
Number of reflections | 58404 | |
<I/σ(I)> | 21.4 | 2.45 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 4.9 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 308 | 2.8M sodium acetate, 0.1M Bis-tris Propane, pH 7.0 |