5GP1

Crystal structure of ZIKV NS5 Methyltransferase in complex with GTP and SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	190
Detector technology	PIXEL
Collection date	2016-06-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.978
Spacegroup name	P 61
Unit cell lengths	123.770, 123.770, 119.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.892 - 2.444
R-factor	0.2389
Rwork	0.237
R-free	0.28030
RMSD bond length	0.003
RMSD bond angle	0.600
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.440
Number of reflections	38127
<I/σ(I)>	12.3
Completeness [%]	99.1
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1M (NH4)2SO4, 10% PEG 8000, 0.1M SPG buffer