5GMD
Crystal structure of Sulfolobus solfataricus diphosphomevalonate decarboxylase in complex with ATP-gamma-S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-11-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 151.238, 151.238, 104.416 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.500 - 1.500 |
| R-factor | 0.16623 |
| Rwork | 0.165 |
| R-free | 0.19234 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4z7c |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.956 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.500 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 72082 |
| <I/σ(I)> | 37.1 |
| Completeness [%] | 99.2 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 293 | 0.1M sodium acetate, 1.5M ammonium sulfate |
