5GKV

Crystal Structure of a Novel Penicillin-Binding Protein (PBP) Homolog from Caulobacter crescentus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.919805
Spacegroup name	P 21 21 21
Unit cell lengths	58.277, 67.308, 92.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.144 - 1.901
R-factor	0.1726
Rwork	0.170
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zyt
RMSD bond length	0.007
RMSD bond angle	0.857
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Rmerge	0.107
Number of reflections	29140
<I/σ(I)>	34.8
Completeness [%]	100.0
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	50mM potassium phosphate monobasic, 20%(w/v) polyethylene glycol 8,000