5GKR
Crystal structure of SLE patient-derived anti-DNA antibody in complex with oligonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-09 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 147.515, 147.515, 112.807 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.330 - 2.100 |
| R-factor | 0.16359 |
| Rwork | 0.163 |
| R-free | 0.18512 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qhk 4y5y 4idj and 4LLW |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 53509 | |
| <I/σ(I)> | 23.1 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl (pH 7.0), 0.2 M NaCl, 1 M Sodium citrate |
