5GJS

Crystal structure of H1 hemagglutinin from A/California/04/2009 in complex with a neutralizing antibody 3E1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97914
Spacegroup name	H 3 2
Unit cell lengths	130.162, 130.162, 365.853
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.980 - 2.900
R-factor	0.2536
Rwork	0.251
R-free	0.30350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lzj 3nfs
RMSD bond length	0.011
RMSD bond angle	1.353
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.160	0.800
Number of reflections	25201
<I/σ(I)>	12.9	2.6
Completeness [%]	93.8	100
Redundancy	6.5	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M MES and 16% PEG 550MME