5GIO

Crystal structure of box C/D RNP with 12 nt guide regions and 13 nt substrates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	P 41 21 2
Unit cell lengths	241.781, 241.781, 145.002
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.957 - 3.604
R-factor	0.2579
Rwork	0.256
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pla
RMSD bond length	0.005
RMSD bond angle	0.885
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.660
High resolution limit [Å]	3.600	3.600
Rmerge	0.165
Number of reflections	49349
<I/σ(I)>	12.4
Completeness [%]	99.7
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	2.0M ammonium sulfate, 2%(v/v) PEG400, 10mM magnesium chloride, 0.1M HEPES