5GHW

Crystal structure of broad neutralizing antibody 10E8 with long epitope bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-01
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	I 2 2 2
Unit cell lengths	66.633, 81.231, 254.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.400 - 2.400
R-factor	0.19366
Rwork	0.192
R-free	0.23041
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g6f
RMSD bond length	0.011
RMSD bond angle	1.431
Data reduction software	MOSFLM (7.1.0)
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.400	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.046	0.202
Number of reflections	24652
<I/σ(I)>	34	8
Completeness [%]	90.0	56.2
Redundancy	9.7	7
CC(1/2)	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	2.5 mM DPC 50% MPD 200 mM phosphate 100 mM TRIS