5GHA
Sulfur Transferase TtuA in complex with Sulfur Carrier TtuB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.28251, 1.28311, 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.139, 93.873, 97.532 |
| Unit cell angles | 109.21, 104.57, 106.86 |
Refinement procedure
| Resolution | 47.840 - 2.502 |
| R-factor | 0.1981 |
| Rwork | 0.196 |
| R-free | 0.24650 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.678 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.840 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 105373 |
| <I/σ(I)> | 16.24 |
| Completeness [%] | 94.9 |
| Redundancy | 1.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M HEPES-NaOH, 0-20%(v/v) PEG6000 |
