5G5S
Structure of the Argonaute protein from Methanocaldcoccus janaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.477, 104.932, 115.077 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.120 - 2.290 |
| R-factor | 0.2112 |
| Rwork | 0.210 |
| R-free | 0.23620 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Phasing software | CRANK |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 1.430 |
| Number of reflections | 34953 | |
| <I/σ(I)> | 18.8 | 1.19 |
| Completeness [%] | 1.0 | 1 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRI- SODIUM CITRATE, 8 % (W/V) POLYETHYLENE GLYCOL 4000 AND 10-12 % (V/V) ISOPROPANOL |
