5G1X
Crystal structure of Aurora-A kinase in complex with N-Myc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-20 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.520, 86.520, 92.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.929 - 1.720 |
| R-factor | 0.1771 |
| Rwork | 0.176 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ceg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.043 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.930 | 1.760 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.060 | 0.740 |
| Number of reflections | 42854 | |
| <I/σ(I)> | 19.8 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.7 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 100 MM BICINE PH 9.0, 20% PEG 6000 |
