5G1S
Open conformation of Francisella tularensis ClpP at 1.7 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 98.226, 127.924, 353.843 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 176.920 - 1.700 |
R-factor | 0.20608 |
Rwork | 0.204 |
R-free | 0.24195 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | OPEN CONFORMATION OF FRANCISELLA TULARENSIS CLPP AT 1.9 A |
RMSD bond length | 0.008 |
RMSD bond angle | 1.299 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.080 | 0.420 |
Number of reflections | 460688 | |
<I/σ(I)> | 9.3 | 1.4 |
Completeness [%] | 94.8 | 70.2 |
Redundancy | 5.7 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | RATIO 1:1, 293K, HANGING DROP. RESERVOIR CONDITION: 0.2 M NACL, 30% (V/V) MPD AND 0.1 M SODIUM ACETATE PH 4.6 |