5G1P
Aspartate transcarbamoylase domain of human CAD bound to carbamoyl phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.670, 157.670, 83.540 |
| Unit cell angles | 90.00, 120.08, 90.00 |
Refinement procedure
| Resolution | 42.540 - 3.190 |
| R-factor | 0.22226 |
| Rwork | 0.221 |
| R-free | 0.25069 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3csu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.638 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.170 | 0.780 |
| Number of reflections | 29277 | |
| <I/σ(I)> | 9.9 | 2.3 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS PH 8.5, 6% PEG 8000, 11% ETHYLENE GLYCOL |
