5G1D
The complex structure of syntenin-1 PDZ domain with c-terminal extension
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.525, 88.529, 90.368 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.810 |
| R-factor | 0.19624 |
| Rwork | 0.194 |
| R-free | 0.23493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | 0.290 |
| Number of reflections | 13799 | |
| <I/σ(I)> | 24 | 4.36 |
| Completeness [%] | 97.6 | 93.9 |
| Redundancy | 8.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M LITHIUM SULFATE MONOHYDRATE, 0.1 M TRIS HYDROCHLORIDE (PH 8.5), 30% (W/V) POLYETHYLENE GLYCOL 4000 |
