5G1B
Bordetella Alcaligenes HDAH native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 100.660, 100.660, 175.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.560 - 1.700 |
| R-factor | 0.15371 |
| Rwork | 0.152 |
| R-free | 0.19147 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g0x |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.508 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.600 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.130 | 0.850 |
| Number of reflections | 99299 | |
| <I/σ(I)> | 8.4 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.25 | pH 5.25 |
