5FY1
Crystal structure of JmjC domain of human histone demethylase UTY in complex with N08619b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-23 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.710, 110.080, 118.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.193 - 1.780 |
| R-factor | 0.2031 |
| Rwork | 0.201 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zli |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | DIMPLE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.190 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.080 | 1.500 |
| Number of reflections | 114896 | |
| <I/σ(I)> | 14.7 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 0.1M HEPES PH 7.2 -- 25% PEG3350 |
