5FY0

Crystal structure of JmjC domain of human histone demethylase UTY in complex with L-malate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-23
Detector	DECTRIS PILATUS 2M
Spacegroup name	P 21 21 21
Unit cell lengths	91.610, 110.740, 120.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.587 - 2.140
R-factor	0.1815
Rwork	0.179
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zli
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	DIMPLE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.590	2.200
High resolution limit [Å]	2.140	2.140
Rmerge	0.140	1.500
Number of reflections	68161
<I/σ(I)>	10	1.1
Completeness [%]	99.7	99.9
Redundancy	6.7	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1M BIS-TRIS PH 6.5 -- 25% PEG3350