5FWE

JMJD2A COMPLEXED WITH NI(II), NOG AND HISTONE H4(1-15)R3me2s PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-05
Detector	DECTRIS PILATUS
Spacegroup name	P 21 21 2
Unit cell lengths	100.739, 149.400, 57.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.854 - 2.050
R-factor	0.2357
Rwork	0.234
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ox0
RMSD bond length	0.006
RMSD bond angle	0.779
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.850	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.100	1.000
Number of reflections	54997
<I/σ(I)>	22.4	2.5
Completeness [%]	99.7	99.8
Redundancy	7.9	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	SITTING DROPS, 277 K, 0.15 M POTASSIUM BROMIDE, 30 % W/V PEG 2000 MME, pH 7.5