5FW3
Crystal structure of SpCas9 variant VRER bound to sgRNA and TGCG PAM target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-22 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.520, 67.800, 187.700 |
| Unit cell angles | 90.00, 111.15, 90.00 |
Refinement procedure
| Resolution | 47.983 - 2.700 |
| R-factor | 0.2159 |
| Rwork | 0.214 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4un3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.682 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.980 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.130 | 0.900 |
| Number of reflections | 57691 | |
| <I/σ(I)> | 13.2 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5 0.3M KSCN 15% (W/V) PEG 3400 |
