5FVF

Room temperature structure of IrisFP determined by serial femtosecond crystallography.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	297
Detector technology	CCD
Collection date	2014-02-14
Detector	MPCCD
Wavelength(s)	1.77
Spacegroup name	P 21 21 21
Unit cell lengths	83.330, 97.760, 142.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.241 - 2.750
R-factor	0.182
Rwork	0.176
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vvh
RMSD bond length	0.014
RMSD bond angle	0.661
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.750	2.750
Rmerge	0.220	1.000
Number of reflections	30981
<I/σ(I)>	3.39	0.95
Completeness [%]	100.0	100
Redundancy	159	70

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	3 M AMMONIUM SULFATE, 100MM BICINE, PH 8.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K