5FUC
Biophysical and cellular characterisation of a junctional epitope antibody that locks IL-6 and gp80 together in a stable complex: implications for new therapeutic strategies
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-12 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 249.030, 67.800, 78.160 |
Unit cell angles | 90.00, 104.53, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.2313 |
Rwork | 0.231 |
R-free | 0.28720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p9m VHH IN-HOUSE STRUCTURE |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.860 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.070 | 0.500 |
Number of reflections | 18151 | |
<I/σ(I)> | 20.88 | 3.97 |
Completeness [%] | 99.4 | 96.9 |
Redundancy | 7.5 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M MES, PH 6.5, 14% PEG20K |