5FTU

Tetrameric complex of Latrophilin 3, Unc5D and FLRT2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS
Spacegroup name	I 41 2 2
Unit cell lengths	292.360, 292.360, 291.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	206.730 - 6.010
R-factor	0.2758
Rwork	0.276
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ftt 5afb 4v2c
RMSD bond length	0.007
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.450	6.150
High resolution limit [Å]	6.000	6.000
Rmerge	0.230	1.500
Number of reflections	16120
<I/σ(I)>	0.89	0.89
Completeness [%]	99.8	100
Redundancy	75.7	74.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.5