5FTT
Octameric complex of Latrophilin 3 (Lec, Olf) , Unc5D (Ig, Ig2, TSP1) and FLRT2 (LRR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.961, 141.486, 151.491 |
| Unit cell angles | 90.00, 117.94, 90.00 |
Refinement procedure
| Resolution | 133.830 - 3.400 |
| R-factor | 0.2261 |
| Rwork | 0.225 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 133.830 | 3.470 |
| High resolution limit [Å] | 3.380 | 3.380 |
| Rmerge | 0.150 | 1.500 |
| Number of reflections | 59624 | |
| <I/σ(I)> | 7.5 | 0.5 |
| Completeness [%] | 98.4 | 92.4 |
| Redundancy | 8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6.0 |
