5FS6
Crystal structure of the V243L mutant of human apoptosis inducing factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2015-08-01 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.840, 114.380, 121.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 121.260 - 1.900 |
R-factor | 0.1738 |
Rwork | 0.172 |
R-free | 0.20689 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fs8 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.609 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.200 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.120 | 0.790 |
Number of reflections | 99250 | |
<I/σ(I)> | 7.4 | 1.6 |
Completeness [%] | 99.3 | 97.8 |
Redundancy | 4.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.2 M AMMONIUM ACETATE, 0.1M HEPES, PH 7.5, AND 20% POLY-ETHYLENE GLYCOL 3350 |