5FS6
Crystal structure of the V243L mutant of human apoptosis inducing factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-08-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.840, 114.380, 121.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 121.260 - 1.900 |
| R-factor | 0.1738 |
| Rwork | 0.172 |
| R-free | 0.20689 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fs8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.609 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.200 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.790 |
| Number of reflections | 99250 | |
| <I/σ(I)> | 7.4 | 1.6 |
| Completeness [%] | 99.3 | 97.8 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M AMMONIUM ACETATE, 0.1M HEPES, PH 7.5, AND 20% POLY-ETHYLENE GLYCOL 3350 |
