5FRC

Structure of urate oxidase prepared by the 'soak-and-freeze' method under 42 bar of oxygen pressure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 2 2 2
Unit cell lengths	80.898, 95.351, 104.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.675 - 1.443
R-factor	0.1427
Rwork	0.141
R-free	0.16480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4po6
RMSD bond length	0.006
RMSD bond angle	1.072
Data reduction software	XDS
Data scaling software	XDS (XSCALE)
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.680	1.530
High resolution limit [Å]	1.440	1.440
Rmerge	0.060	0.060
Number of reflections	70982
<I/σ(I)>	11.5	1.5
Completeness [%]	97.5	94
Redundancy	4.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1