5FR1
Double acetylated RhoGDI-alpha in complex with RhoA-GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.100, 67.640, 120.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.110 - 2.750 |
| R-factor | 0.20173 |
| Rwork | 0.199 |
| R-free | 0.25598 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hh4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.674 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.110 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.097 | 0.364 |
| Number of reflections | 11805 | |
| <I/σ(I)> | 9.2 | 3 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 4 M SODIUM FORMATE, 0.1 M SODIUM ACETATE PH 4.5 |
