5FR0
The details of glycolipid glycan hydrolysis by the structural analysis of a family 123 glycoside hydrolase from Clostridium perfringens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 99.530, 99.530, 95.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.310 - 1.750 |
| R-factor | 0.18274 |
| Rwork | 0.181 |
| R-free | 0.22099 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fqe |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.640 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.110 | 0.480 |
| Number of reflections | 55369 | |
| <I/σ(I)> | 14.7 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.6 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
