5FQO
Crystal structure of M. musculus protein arginine methyltransferase PRMT6 with SAH and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-17 |
| Detector | DECTRIS PILATUS 3K |
| Spacegroup name | I 41 |
| Unit cell lengths | 78.620, 78.620, 118.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.702 - 1.899 |
| R-factor | 0.1601 |
| Rwork | 0.158 |
| R-free | 0.19830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c08 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.170 |
| Number of reflections | 27171 | |
| <I/σ(I)> | 22.5 | 4.8 |
| Completeness [%] | 96.4 | 84.4 |
| Redundancy | 5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRIS PH 8.0 200 MM MGCL2 10% PEG 6000 |
