5FQM
Last common ancestor of Gram Negative Bacteria (GNCA) Class A beta- lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 41 |
| Unit cell lengths | 94.230, 94.230, 93.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.070 - 1.500 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.19790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b88 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.390 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.070 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.170 | 0.730 |
| Number of reflections | 59525 | |
| <I/σ(I)> | 3.7 | 1.1 |
| Completeness [%] | 91.3 | 95.7 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2.0M NH4SO4, 0.1M TRIS-HCL PH 8.0 |
