5FQG
The details of glycolipid glycan hydrolysis by the structural analysis of a family 123 glycoside hydrolase from Clostridium perfringens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 113.340, 113.340, 95.951 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 98.160 - 2.300 |
R-factor | 0.22808 |
Rwork | 0.226 |
R-free | 0.27279 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fqe |
RMSD bond length | 0.009 |
RMSD bond angle | 1.282 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.100 | 0.690 |
Number of reflections | 32038 | |
<I/σ(I)> | 16.7 | 4.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15.2 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |