5FPU
Crystal structure of human JARID1B in complex with GSKJ1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-06 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 141.660, 141.660, 151.980 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.601 - 2.240 |
R-factor | 0.1911 |
Rwork | 0.190 |
R-free | 0.22110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a1f |
RMSD bond length | 0.002 |
RMSD bond angle | 0.719 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.460 | 2.300 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.090 | 0.090 |
Number of reflections | 43823 | |
<I/σ(I)> | 4.8 | 24.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20.2 | 19.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.1M HEPES PH 8.0, 0.8M POTASSIUM PHOSPHATE-DIBASIC, 0.8M SODIUM PHOSPHATE MONOBASIC |