5FP8
Crystal structure of human KDM4D in complex with 3-4-methylthiophen-2- ylmethylaminopyridine-4-carboxylic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-09 |
| Detector | RIGAKU SATURN A200 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.286, 71.286, 151.054 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.300 - 1.980 |
| R-factor | 0.1615 |
| Rwork | 0.159 |
| R-free | 0.20638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.080 | 2.080 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.050 | 0.230 |
| Number of reflections | 24530 | |
| <I/σ(I)> | 12.3 | 3.2 |
| Completeness [%] | 88.7 | 84.4 |
| Redundancy | 3.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 100MM HEPES PH 7.4, 2.45M AMMONIUM SULPHATE |
