5FNV
a new complex structure of tubulin with an alpha-beta unsaturated lactone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.160, 157.200, 181.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 118.880 - 2.610 |
| R-factor | 0.18773 |
| Rwork | 0.186 |
| R-free | 0.22555 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i55 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.636 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.490 | 2.680 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 88059 | |
| <I/σ(I)> | 15.8 | 1.6 |
| Completeness [%] | 94.8 | 67.5 |
| Redundancy | 5.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 0.1M MES PH6.7;6% PEG4000 ;5% GLYCEROL;30MM CACL2;30MM MGCL2 |
