5FMP
KstR, transcriptional repressor of cholesterol degradation in Mycobacterium tuberculosis, bound to the DNA operator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-10-23 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.530, 62.781, 98.647 |
| Unit cell angles | 90.00, 89.90, 90.00 |
Refinement procedure
| Resolution | 44.310 - 2.260 |
| R-factor | 0.20539 |
| Rwork | 0.202 |
| R-free | 0.27039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mnl |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.484 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.510 | 2.380 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.060 | 0.250 |
| Number of reflections | 20253 | |
| <I/σ(I)> | 8.9 | 1.7 |
| Completeness [%] | 87.3 | 50.1 |
| Redundancy | 2.4 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.1 | 0.2 M SODIUM SULFATE, 8% TACSIMATE, PH 5.1; 21.7% PEG 6000 |
