5FMH
Crystal structure of the E405K mutant of human apoptosis inducing factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2014-05-31 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.300, 49.230, 81.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.480 - 1.800 |
| R-factor | 0.19321 |
| Rwork | 0.191 |
| R-free | 0.24254 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bv6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.485 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.390 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.130 | 1.020 |
| Number of reflections | 37766 | |
| <I/σ(I)> | 7.2 | 1.1 |
| Completeness [%] | 93.7 | 88 |
| Redundancy | 5.4 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% PEG 3350; 0.2M AMMONIUM ACETATE, pH 7 |
