5FMA
human Notch 1, EGF 4-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-27 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.940, 86.830, 53.450 |
| Unit cell angles | 90.00, 107.58, 90.00 |
Refinement procedure
| Resolution | 43.420 - 2.460 |
| R-factor | 0.2594 |
| Rwork | 0.259 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vj3 |
| RMSD bond length | 0.035 |
| RMSD bond angle | 1.210 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 2.530 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.050 | 0.390 |
| Number of reflections | 12129 | |
| <I/σ(I)> | 11.9 | 2.3 |
| Completeness [%] | 94.1 | 93.8 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
